buffer d (Thermo Fisher)

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Thermo Fisher buffer d
Buffer D, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+d/Triton+X-100/pm42303864-432-53-108
Average 99 stars, based on 1 article reviews

buffer d - by Bioz Stars, 2026-09

99/100 stars

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Incubation:

Article Title: Nuclear export of R-loop by the DDX1 and XPO1 complex promotes senescence-associated secretory phenotype and inflammaging.
Article Snippet: .. Next, the pellet was resuspended in 300 μl buffer C (50 mM HEPES, pH 7.5, 250 mM KCl, 2.5 mM MgCl2 and 0.05% Triton X-100), samples were then centrifuged at 10,000g for 15 min at 4 °C and the supernatant was collected as fraction C. Next, the pellet was resuspended in 300 μl buffer D (50 mM HEPES, pH 7.5, 500 mM KCl, 2.5 mM MgCl2 and 0.1% Triton X-100), samples were then centrifuged at 10,000g for 15 min at 4 °C and the supernatant was collected as fraction D. The remaining pellet was resuspended in buffer B supplemented with 5 mM CaCl2 and 3 U micrococcal nuclease (Thermo Fisher), incubated at 37 °C for 10 min, and the supernatant was collected as chromatin fractionation after centrifugation at 15,000g for 10 min at 4 °C. .. Whole-cell lysates and isolated chromatin using Pierce Chromatin Prep Module (Thermo Fisher, cat. no. 26158) were prepared as controls.

Fractionation:

Centrifugation:

Electron Microscopy:

Article Title: Isolation and Molecular Profiling of Nuclei of Specific Neuronal Types from Human Cerebral Cortex and Striatum
Article Snippet: 31.7 ml DNase/RNase‐Free water 8.3 ml Buffer B dissolve 4 EDTA‐free Protease Inhibitor Cocktail tablets (Roche, cat. no. 04693159001) and chill solution on ice Add 10 ml 1.25 M sucrose solution (final 0.25 M) 50 μl 0.5 M spermidine solution (0.5 mM final) (Sigma Aldrich, cat. no. S2501) 75 μl 0.1 M spermine solution (0.15 mM final) (Sigma Aldrich, cat. no. S2876) 50 μl 1 M dithiothreitol (1 mM final) Thermo Fisher Scientific, cat. no. 165685000) 50 μl SUPERase‐In RNase Inhibitor (Thermo Fisher Scientific, cat. no. AM2696) 50 μl Recombinant RNasin Ribonuclease Inhibitor (Promega, cat. no. N2515) Prepare fresh for each experiment, keep on ice .. 935 μl Buffer D 2 μl EDTA (0.5 M), pH 8.0 (Thermo Fisher Scientific. cat. no. AM9260G) 62.5 μl Paraformaldehyde 16% Aqueous Solution (Electron Microscopy Sciences, cat. no. 15710) ..

Protease Inhibitor:

Article Title: Application of a novel RNA-protein interaction assay to develop inhibitors blocking RNA-binding of the HuR protein
Article Snippet: .. Next, 5 μL of streptavidin-tagged SmBiT (Promega) was combined with 5 μL of the RNA oligo in 300 μL of Buffer D (20 mM Tris, pH 7.9, 20% glycerol, 0.1 M KCl, 0.2 mM EDTA, and 0.5 mM DTT) containing RNase inhibitor (RiboLock) and protease inhibitor (ThermoFisher). ..

Binding Assay:

Article Title: Expanding the genetic code with diverse backbone structures across diverse sequence contexts
Article Snippet: The mixture was centrifuged 4 °C, 4000 rcf for 10 min. 200 μL of the aqueous phase were transferred into a new Eppendorf tube and RNA was purified with a Monarch RNA Cleanup Kit (50 μg) (NEB). .. RNA was eluted in 50 μL water into an Eppendorf tube containing 50 μL 2x Buffer D. Ninety microlitres of sample were hybridised with 0.5 μL of biotinylated DNA probe (100 μM) and annealing was carried out at 65 °C for 5 min. For each sample 40 μL of Dynabeads MyOne C1 streptavidin beads (Invitrogen) were washed twice with Buffer D +0.05% (v/v) Tween 20 and resuspended in 10 μL Buffer D. Beads were added to each sample and binding was carried out at 4°C with head-over-tail rotation for 1 h. Samples were washed with 3× acW1-t, 2× acW1, 3× acW2 and 1× water. ..

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